Journal: Frontiers in Pharmacology
Article Title: Phosphoglycerate kinase 1 as a potential prognostic biomarker in papillary thyroid carcinoma
doi: 10.3389/fphar.2025.1542159
Figure Lengend Snippet: PGK1 is expressed strongly in cancerous tissue, but weakly in adjacent tissues. (A) lmmunohistochemical images of tissue microarray (TMA) analysis for PGK1 from a paired cancerous (a) and adjacent non-cancerous (b) case (magnifcation, ×50). Scale bar: 100 μm. Zoom image for PGK1 of a paired cancerous (c) and adjacent (d) case (magnifcation, ×400). Scale bar: 50 μm. (B) Positive ratio of PGK1 staining in TMA, 79.3% (46 of 58 cases) in cancerous samples and 18.9% (11 of 58 cases) in adjacent samples. (C) Quantitative RT-PCR analysis of the expression level of PGK1 in two groups. (D) Immunoblot of the protein level of PGK1 in two groups. (E) Immunoblot analysis of the protein level of PGK1 in two groups. Data are expressed as mean ± SEM. ** P < 0.01, *** P < 0.001.
Article Snippet: A rabbit monoclonal antibody against human PGK1 (proteintech, United States, 17811-1-AP) was introduced and incubated at 4°C overnight.
Techniques: Microarray, Staining, Quantitative RT-PCR, Expressing, Western Blot